Journal: Cells
Article Title: Engineered Pullulan-Collagen-Gold Nano Composite Improves Mesenchymal Stem Cells Neural Differentiation and Inflammatory Regulation
doi: 10.3390/cells10123276
Figure Lengend Snippet: Identification of various nanomaterials by FTIR, SERS, and XPS assay. ( A ) The FTIR spectra evaluated the specific functional groups. The combination of Pul and Col led to the shift of C–O–C vibration from 929 cm −1 to 910 cm −1 , C–O vibration from 1029 cm −1 to 1033 cm −1 , CH 2 /CH 3 functional group from 2931 cm −1 to 2935 cm −1 , and amide II band from 1540 cm −1 to 1550 cm −1 . N-H stretching at 3340 cm −1 was also found in the Pul–Col–Au group, indicating Au nanoparticles successfully bonding with Pul–Col. ( B ) The Raman shift spectra measured in the region from 1100 cm −1 to 2400 cm −1 by SERS. The specific absorption peaks of Pul–Col–Au were detected at 1259 cm −1 (amide III), 1347 cm −1 (CH 2 /CH 3 ), 1470 cm −1 (CH 2 /CH 3 ), and 1688 cm −1 (amide I), indicating the presence of Pul, Col, and Au. ( C ) The wide-scan XPS spectra of various nanomaterials. The combination of Pul and Col shows the N1s peak position at 402.6 eV binding energy due to the amide group in Col. The binding energy of Au at 86 and 91.8 eV was detected in Pul–Col–Au, indicating the structure of Pul–Col would not change by the addition of Au.
Article Snippet: A Raman microscopic system (LABRAM HR UVVIS−NIR Version, HORIBA Ltd., Kyoto, Japan) was used to investigate the chemical structure of the nanomaterials.
Techniques: Functional Assay, Binding Assay